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WILDkCAT is a Python package that offers a comprehensive range of functions for extracting, retrieving and predicting enzyme kinetic data for a given metabolic model. The tool facilitates enzyme-constrained modelling by assigning specific kcat values to each combination of reaction, enzyme, and substrate(s).

WILDkCAT integrates the BRENDA and SABIO-RK APIs to retrieve experimental values and CataPro to predict missing values. It can be used with various enzyme-constrained metabolic modelling methods, such as COBREXA.jl and GECKO, facilitating the seamless integration of kcat values into a given metabolic model.